Whole genome mapping by DNA sequencing with linked paired-end library
Overview
Executive Statement:
A novel and highly efficient method of sequencing and mapping the whole genome, promising increased accuracy and lower costs.
Description:
This technology addresses challenges in genomics like accurate de novo genome assembly and structural variant analysis. It proposes a more efficient approach to sequencing and genome mapping using a method of generating linked-paired-end nucleic acid fragments from a DNA sample. The method promises to offer a better resolution of haplotypes, reduce the complexity, time, and cost of sequencing projects, and enhance the detection and analysis of structural variations in the genome.
Market Applications
- Whole genome sequencing for research, clinical, and medical applications
- Genetic testing and personalized medicine
- Research in infectious and autoimmune diseases
- Biotechnological applications in agriculture, animal breeding, etc.
Key Advantages
- Greater accuracy in genome assembly and structural variant analysis
- Improved efficiency and reduced complexity of the sequencing project
- Reduced time and cost
- Enhanced detection of structural variations
Problems Solved
- Challenges in cloning large DNA fragments
- Difficulty in sequencing highly repetitive sequences
- Issues with positional information regarding the locations of copy number variant
Intellectual Property and Development Status
US Patent Issued #US9758780B2 - Whole genome mapping by DNA sequencing with linked-paired-end library
References
Nature Scientific Reports 2024: A long-read sequencing strategy with overlapping linkers on adjacent fragments (OLAF-Seq) for targeted resequencing and enrichment.
Commercialization Opportunities