Methods of specifically labeling nucleic acids using CRISPR/Cas9
Overview
Executive Statement:
A new genome analysis technique that uses guide RNA and Cas9 nickase to detect target nucleic acid sequences using fluorescent labeling.
Description:
The technology involves a method for sequence-specific labeling that can target repetitive regions in the genome. This method contacts genomic DNA with a guide RNA and Cas9 nickase to produce a single-strand break (nick) adjacent to the target sequence. A fluorescently labeled nucleotide is incorporated into the nicked DNA, which is then used to detect the target nucleic acid sequence. This technique can be used for genomic research, clinical diagnostics, identification of structural variants, repetitive region mapping, etc.
Market Applications
- Clinical diagnostics for detecting and typing structural variations
- Development of new genome editing tools and techniques
- Genomic research and analysis
- Clinical Diagnostics
Key Advantages
- Allows for precise mapping of long-range de novo assembly contiguity and validation
- Can target repetitive regions often lacking in restriction site motif
- Capable of detecting specific structural variations, providing accurate breaking points
- Offers a potential solution to sequence mis-assembly in complex, duplicated, and repetitive regions
- Improved accuracy in detecting target nucleic acid sequence
- Creates a barcode of a portion of the genomic DNA
Problems Solved
- Inadequate long-range de novo assembly contiguity
- Sequence mis-assembly in complex segmentally duplicated and repetitive regions
- Detection and delineation of structural variations
- Overcomes the limitation of being unable to target repetitive regions
Intellectual Property and Development Status
US Patent Issued #US10640810B2 - Methods of specifically labeling nucleic acids using CRISPR/Cas - Expires 2037
References
Nature Scientific Reports 2024: A long-read sequencing strategy with overlapping linkers on adjacent fragments (OLAF-Seq) for targeted resequencing and enrichment
Nucleic Acids Research 2015: CRISPR-CAS9 D10A nickase target-specific fluorescent labeling of double strand DNA for whole genome mapping and structural variation analysis
Commercialization Opportunities