Single molecule real-time long DNA mapping and sequencing
Overview
Executive Statement:
The invention offers an innovative way to immobilize and optically map/sequence oligonucleotides on a micropatterned substrate for efficient large-scale genomic assembly.
Description:
This technology presents a method for immobilizing and linearizing oligonucleotides on a micropatterned substrate. It also describes its associated chemistry for mapping and sequencing DNA. The invention addresses the need for new devices and methods to stretch single DNA molecules and to immobilize and linearize oligonucleotides for efficient large-scale genomic assembly.
Market Applications
- Genome mapping and sequencing
- Diagnostic testing for DNA-based diseases
- Biomedical research
Key Advantages
- Efficient and accurate mapping/sequencing of DNA sequences
- Ability to generate super-long DNA sequencing and mapping reads
- Resolves the limitations of traditional mapping and sequencing technologies
Problems Solved
- Labor-intensive and resource-heavy traditional DNA mapping and sequencing
- Limited length of linearized DNA for efficient large-scale genome assembly
Intellectual Property and Development Status
United States Patent Pending- Publication #US20210033606A1 - DNA mapping and sequencing on linearized DNA molecules
References
Nature Scientific Reports 2019: A micropatterned substrate for on-surface enzymatic labelling of linearized long DNA molecules
Commercialization Opportunities