Multicolor whole-genome mapping for genetic analysis
Overview
Executive Statement:
A novel method for precise genome mapping and sequencing using Cas9-mediated labeling within nanochannels.
Description:
This technology is a method for mapping a whole genome using a universal multi-color mapping strategy in nanochannels that combines a conventional sequence-motif labeling system with Cas9-mediated target-specific labeling. This technique allows the detection of structural variants and enables custom labels to interrogate features not accessible to motif labeling, locate breakpoints, and precisely estimate copy numbers of genomic repeats.
Market Applications
- Detection and analysis of genetic disorders and pathogenic conditions
- Detailed genome mapping in research and clinical settings
- Investigating mutagenesis and quantifying copy numbers
Key Advantages
- Ability to detect features not accessible to motif-labeling
- Precise location of breakpoints
- Estimation of copy numbers of genomic repeats
- Cost-effective alternative to long-read sequencing technologies
Problems Solved
- Difficulty in characterizing structural variants with short-read sequencing
- Inability to precisely locate breakpoints or estimate copy numbers
Intellectual Property and Development Status
Patent Pending #WO2022266464A1 - Multicolor whole-genome mapping and sequencing in nanochannel for genetic analysis
Canada Patent Pending #CA3223202A1 - Multicolor whole-genome mapping and sequencing in nanochannel for genetic analysis
References
Nature Scientific Reports 2020: Multiplex structural variant detection by whole-genome mapping and nanopore sequencing
Nucleic Acids Research 2021: Customized optical mapping by CRISPR–Cas9 mediated DNA labeling with multiple sgRNAs
Commercialization Opportunities