Quantification of On-off target gene editing by optical mapping
Overview
Executive Statement:
A revolutionary methodology for quantifying on-target and off-target gene editing activity using CRISPR-Cas9 and optical mapping.
Description:
This technology comprises a single-molecule optical mapping strategy using CRISPR-Cas9 to fluorescently label the modified genes. The method involves scanning the whole genome, locating molecules with modified genes, and quantifying gene modification events for both on-target and off-target gene modification.
Market Applications
- Potential to revolutionize gene editing research in genomics
- Useful in therapeutics for genetic diseases where precise gene editing is required
- Could be essential in agricultural biotechnology for crop improvement
Key Advantages
- Provides an efficient, less time-consuming, and less costly alternative to Whole Genome Sequencing (WGS)
- Allows for unbiased detection and true off-target gene editing frequency
- Capable of quantifying less than 1% of gene modification events
Problems Solved
- Solves the inefficient, expensive, and impractical nature of Whole Genome Sequencing (WGS)
- Overcomes the limitations of biased detection in existing target enrichment sequencing protocols
Intellectual Property and Development Status
United States Provisional filed
Commercialization Opportunities